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Showing posts with label Testicular Sperm Extraction (TESE). Show all posts
Showing posts with label Testicular Sperm Extraction (TESE). Show all posts

Azoospermia is not a big deal.




Azoospermia is a severe male factor in the infertility, and the occurrence is around 15% in the infertile men. There are two types of azoospermia,—obstructive and non-obstructive.
Meanwhile, we can classify Azoospermia more detail into three major types as listed.

1. Non-obstructive
2. Hormone deficiency
3. Testicular failure

Literally, non-obstructive azoospermia refers to no sperm in the semen because of abnormal sperm production.
The fertility specialist generally recommended these patients to take microsurgical epididymal sperm aspiration (MESA) or testicular sperm extraction (TESE) combing with intracellular single sperm injection (ICSI) in the IVF program.

How to do the MESA/TESE?

Under regional anesthesia, we would cut apart the scrotum and use a surgical microscope to open the small tubes within the epididymis to look for mature sperm or immature sperm cells. The sperm harvested can be used immediately or frozen for use at a later time. If no sperm are found in epididymis, it is necessary to look inside the testicle for viable sperm.
Usually, the number of sperm retrieved is small and poor activity. Therefore, it is very precious. It is best to use intracellular single sperm injection (ICSI) to increase the fertilization rate. The remaining sperm can be frozen for future use.

This is the most effective solution to solve azoospermia retrieving sperm.
Also, with the Vitrification, it can be used several times at a time to achieve maximum benefit.
Through MESA/TESE and ICSI, it helps to increase the fertilization rate by approximately 70%. About the pregnancy rate, it’s the same as normal in vitro fertilization which is 50%-60%.




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Stork Fertility Center Stork Fertility Center Author

Hyaluronan(HA) binding assay: a good matchmaker to sperm



For in-vitro fertilization, the oocytes attract the sperms with better maturation status by hyaluronic acid of the surrounding cumulus cells.






In the 2016 annual meeting of Taiwan Society of Reproductive Medicine, Dr. Dionisios Sakkas from Boston IVF center, US, shared his experiences in semen processing and sperm selection,

1. Density:Gradient separation 

2. Surface Charge:Electrophoresis 


4. Motility Characteristics: Microfluidics

5. Membrane Integrity:Hyaluronan(HA)-binding

6. Surgical:MESA/TESE 


Stork Fertility Center Stork Fertility Center Author

Azoospermia



Male factor is one of the important issues in the infertility. Azoospermia is one of the tricky things in the male infertility. If a problem occurred in the procedure of spermatogenesis (sperm production) or sperm transport, it results in azoospermia, which means no sperms found in the ejaculated semen.


Stork Fertility Center Stork Fertility Center Author

Are you afraid of testicular sperm extraction (TESE)?


Before starting the IVF program, the diagnostic microdissection testicular sperm extraction can improve the success rate of surgical sperm extraction on the oocyte retrieval day. It would be a great news in the couples with azoospermia.

Stork Fertility Center Stork Fertility Center Author

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