Stork Diary.
to Share Stork Life Events

Lab Touring - PGS, Preimplantation Genetic Screening by next-generation sequencig (NGS)

              The geneticist is operating the NGS platform at Stork Fertility Center.

The traditional prenatal examinations include chorionic villus sampling (CVS) and amniocentesis. However, the induction would be required if either CVS or amniocentesis revealed significant chromosomal abnormalities, and it becomes psychological stress. Perimplantation Genetic Screening(PGS)is an advanced technology, combining IVF and cell molecular biology, taking the third day embryo (about 6-10 cells now) or the 5th day blastocyst cell for chromosome analysis. The widely used analysis platform is based on array comprehensive hybridization (aCGH), but the recent researches reported that the next-generation sequencing platform performs better resolution and sensitivity. In addition, the low-rate aneuploidy is displayed more clearly in the NGS than in the aCGH.


Technique
NGS
aCGH
Resolution
Diagnosed range: affected length>10Mb
Detected range: affected length >2Mb
Diagnosed range: affected length>10Mb
Application
1. Frequent early abortion
2. Repeated failure in IVF
3. Advanced maternal age
4. Undefined infertility
5. Severe male infertile factor
Embryo stage

D5/D6 good blastocyst (BB)

 




Accuracy
90%
90%
Embryo transfer
Warmly thawed embryo transfer
Prenatal exam
Amniocentesis  is recommended
Amniocentesis  is recommended


The aneuploid rate increases with the maternal age.
 


The general exam procedure of PGS is showed in the following figure,



  •  
Stork Fertility Center送子鳥 Stork Fertility Center送子鳥 Author

1 comment:

  1. Want to get all the details on Preimplantation Genetic Screening then you need to follow this blog link. We provide you all the details over this blog post.

    ReplyDelete